Postdoc in Biochemistry and structural biology of cholesterol transport proteins for the discovery of new small molecule inhibitors

 

A postdoc fellowship in protein biochemistry and structural biology funded by the Novo Nordisk Foundation is available at the Department of Chemistry at The Technical University of Denmark (DTU). The project will be under the supervision of Associate Professor Luca Laraia and will start on September 1st 2021 or as soon as possible thereafter. The successful candidate will join a young research team working at the interface of chemistry and biology.

Responsibilities and qualifications
The successful applicant for this position will become a vital element of the research programme of the laboratory. Their work should lead to the establishment of a biophysical and biochemical assay platform needed for identifying and validating inhibitors of cholesterol transport proteins. The project will be carried out in close collaboration with the group of Professor J. Preben Morth, based at the department for Biotechnology and Biomedicine at DTU.

Candidates should have a PhD in biochemistry or related discipline together with a knowledge of biochemistry and biophysical methods such as dynamic scanning fluorimetry, fluorescence polarization and FRET. Thorough knowledge of protein expression and purification will be necessary and it will be advantageous to have general knowledge of bacteriological methods, cloning, protein engineering and x-ray crystallography. The candidate should be highly motivated, able to work independently and should be confident in both written and spoken English. The applicant should strive towards scientific excellence, be ambitious and hard working.

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and terms of employment
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The position is available for 24 months in the first instance and will start on September 1st  2021 or as soon as possible thereafter.

You can read more about career paths at DTU here.

Further information
Further questions about the project should be directed to Luca Laraia, email: luclar@kemi.dtu.dk or phone: 0045 45252495

Please do not send applications to this e-mail address, instead apply online as described below.

If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark.

Application
Please submit your online application no later than 2 July 2021 (Danish time).

Please note that applicants will be evaluated on an ongoing basis.

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

• Application (cover letter)
• CV (including a list of publications and contact details for 2-3 references)
• Academic Diplomas (MSc/PhD)
• List of publications

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,900 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. DTU has campuses in all parts of Denmark and in Greenland, and we collaborate with the best universities around the world.

Apply for this job

Apply no later than 2 July 2021
Apply for the job at DTU Chemistry by completing the following form.

Apply online

Research Assistant in Whole genome sequencing of bacteria producing natural products

 

Are you our new colleague interested to contributing to find the next generation of antibiotics from actinomycetes?

We are seeking a highly motivated Research Assistant as a maternity cover to join our international team at the Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain), Technical University of Denmark, and become part of the Natural Products Genome Mining group and the NNF Challenge Project “Integration of Informatics and Metabolic Engineering for the discovery of novel antibiotics (iimena)”.

Responsibilities and qualifications
We are currently in the process of sequencing many natural products producing bacteria from our own strain collection and from collections of collaboration partners. This requires the cultivation of hundreds of strains and preparation of numerous high-quality chromosomal DNA extractions and sequencing libraries. You will therefore complement our sequencing team with your expertise.

Your primary tasks will be

• Maintaining microbial strains and documenting them in our LIMS
• Cultivation of actinomycetes and high-quality chromosomal DNA isolation
• DNA quality control
• Oxford Nanopore library construction, quality control and sequencing
• Sequence data analysis, including assembling and annotating the data
• Support with routine lab duties, such as preparing media, coordinating with the DTU Biosustain sequencing lab/external sequencing providers

As a formal qualification, you must have a master’s degree in biology/biotechnology or equivalent.

Further competences that are important for the position are:

• Previous experience in molecular techniques working with bacteria; experience in working with “difficult” non-model organisms, especially actinomycetes, is advantageous.
• Previous experience with molecular biotechnology techniques like sequencing library preparation, genome sequencing (Oxford Nanopore is advantageous), DNA isolation and purification
• Great attention to details
• Excellent interpersonal, networking and communication skills for working in a highly international research environment
• Strong English written and oral language skills, as our work language is English

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

The Natural Products Genome Mining group at DTU Biosustain is a highly dynamic team of technicians, research assistants, PhD students, postdocs, researchers, and Professor Tilmann Weber. We work closely together to achieve our ambitious goals in creative and interesting ways by using, improving, and developing technologies such as nanopore sequencing, BGC cloning, high throughput screening, CRISPR base editing, and the antiSMASH BGC prediction platform.

Salary and terms of employment
The appointment will be based on the collective agreement with the Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The position is limited to 28.2.2023

You can read more about career paths at DTU here.

Further information
Further information may be obtained by email from Prof. Tilmann Weber, email: tiwe@biosustain.dtu.dk

You can read more about DTU Biosustain at www.biosustain.dtu.dk.

If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark.

Application procedure
Your complete online application must be submitted no later than 4 July 2021 (Danish time).

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

• Application (cover letter)
• CV
• Academic Diplomas (MSc)

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

The Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain)
Recent progress in our ability to read and write genomic code, combined with advances in automation, analytics and data science, has fundamentally changed the scope and ambition of harnessing the potential of biological systems. Big data approaches and analysis of biological systems are key research instruments at the Center. DTU Biosustain utilizes these advances for microbial cell factory design to foster sustainable lifestyles in relation to three application areas: Sustainable Chemicals, Natural Products, and Microbial Foods.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,900 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. DTU has campuses in all parts of Denmark and in Greenland, and we collaborate with the best universities around the world.

Apply for this job

Apply no later than 4 July 2021
Apply for the job at Center for Biosustainability by completing the following form.

Apply online

Postdoctoral researcher in Ubiquitin Signalling (biochemistry and mechanisms of stress and inflammation signalling)

 

A 2-year postdoc position in ubiquitin signalling is available from 1 September 2021 or shortly thereafter (starting date can be negotiated) in the laboratory of Associate Professor Rune Busk Damgaard .The project is funded by the Novo Nordisk Foundation and the successful candidate will join an ambitious and international research team at the Department of Biotechnology and Biomedicine, Technical University of Denmark.

Research in the Ubiquitin Signalling and Inflammation Lab aims at understanding how cell signalling, particularly at the interface of inflammation, cell death, and metabolism, is regulated by ubiquitin modifications and how dysregulation of these signalling mechanisms leads to disease development, including autoinflammatory diseases and cancer. A particular focus of the lab is to explore and understand the cellular functions of the enigmatic 'atypical' ubiquitin chains. Our long-term goal is to discover new signalling mechanism and identify molecular processes that can be targeted pharmacologically to treat cancer and other diseases caused by chronic or dysregulated inflammation.

Responsibilities and qualifications
The postdoc will pioneer the development of new tools for deciphering atypical ubiquitin chain signalling, including M1-linked/linear chains, to understand how these ubiquitin modifications control cell signalling.

The project will involve experimental laboratory work using nanobody discovery and phage display technologies, molecular cloning, protein biochemistry, recombinant protein expression, in vitro assays, molecular cell biology, and cell culture systems to develop new techniques and tools to study the function of the enigmatic atypical ubiquitin modifications in cells. The project may also include cutting-edge mass spectrometry-based proteomics analyses performed in collaboration with the DTU Proteomics Core.

We are looking for a talented, curious, and highly motivated a candidate with the following qualifications and experience for this ambitious and pioneering project:

Essential skills:

• PhD degree in biochemistry, molecular biology, or similar field
• Strong background in biochemistry, molecular biology, and experimental design / assay development
• Solid, demonstrated experience in protein biochemistry, including recombinant protein design, expression, and purification
• Demonstrated experience in molecular biology, including PCR and molecular cloning, gel electrophoresis, and DNA sequencing
• Demonstrated experience in in vitro enzymatic assays

Desirable skills:

• Experience with ubiquitin signalling, protein quality control, or cell stress
• Experience with analysis of cell signalling and multiprotein complexes (e.g. immunoprecipitation and pulldowns, western blotting, etc.)
• Experience with phage display technologies or nanobody discovery
• Experience with mammalian cell culture and cell biology
• Experience with flow cytometry
• Experience with genome editing (e.g. CRISPR/Cas9)
• Experience with mass spectrometry-based proteomics

The successful candidate should thrive in a highly ambitious research group and will have demonstrated the ability to work independently and a documented track record of publication in internationally recognised peer-reviewed journals. Good communication skills in both written and spoken English are a requirement.

We offer
The Ubiquitin Signalling and Inflammation Lab offers a highly ambitious, exciting, encouraging, and dynamic work environment. Together, we design and execute experiments that lead to discovery of new signalling mechanisms involved in development of inflammatory disease and cancer. We work together in a young and fun team. We grow together as scientists to tackle important, fundamental research questions. Read more about the lab and our research here: http://bioengineering.dtu.dk/usi

The Ubiquitin Signalling and Inflammation lab is part of the Department of Biotechnology and Biomedicine, Technical University of Denmark. The department is highly interdisciplinary and a leading department within life sciences in Denmark, covering both fundamental, technological, and applied aspects of biomedical research. The successful candidate will join a recently started lab with excellent funding and support from Novo Nordisk Foundation, Lundbeck Foundation, and DTU. The candidate will benefit from a thriving international and collaborative community and state-of-the-art facilities with access to cutting-edge technologies and equipment.

DTU is a leading technical university globally recognised for the excellence of its research, education, innovation, and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterised by collegial respect and academic freedom tempered by responsibility.

Assessment
The assessment of the applicants will be made by Associate Professor Rune Busk Damgaard together with relevant project partners. Applications will be assessed on a rolling basis.

Salary and terms of employment
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

This is a full-time position. The start date is 1 September 2021 or as negotiated. The position is for 2 years in the first instance. The maximum employment period as a postdoc is 4 years.

The laboratory is located at DTU Lyngby Campus (Greater Copenhagen, Denmark).

Further information
Informal enquiries and requests for further information should be directed to Associate Professor Rune Busk Damgaard (rudam@dtu.dk)

If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark.

Application procedure
Please submit your online application no later than 12 July 2021. 

Applications must be submitted as a single PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill in the online application form, and attach all your materials in English in one PDF file. The file must include:

• Application (cover letter)
• CV
• List of publications
• Academic diplomas (PhD, or MSc diploma if PhD degree has not been conferred)
• Names and contact details for two academic references

Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, race, disability, religion or ethnic background are encouraged to apply.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,900 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. DTU has campuses in all parts of Denmark and in Greenland, and we collaborate with the best universities around the world.

Apply for this job

Apply no later than 12 July 2021
Apply for the job at DTU Bioengineering by completing the following form.

Apply online